Chromatography of Dyes :: Chromatography Experiments Dye Essays

Chromatography of DyesAn extremely common technique by using two chemicals and filter make-up can give you sufficient information. A well-done Chromatography of dyes will provide you with enough data to determine if a liquid mixture is heterogeneous or homogenous . In a learning activity you can depart and possibly identify food dyes in some subscribes mixes and food colors. The effect of effect polarity or the ability to separate and elute compounds will also be demonstrated. In this investigate you will need to use the equation Xcm/Acm=RF value. The length of A, is the length of the longest line of the food dye or food coloring. The Length of X is the length of all the other variables. In the experiment the lengths will be measured in Centimeters.Experimental ProcedureYou will need to obtain a piece of standard filter authorship somewhat 10*15 cm. Use a pencil to draw a line parallel to one of the longer edges, about 1 cm from the edge. put one over 10 dashes on the pencil line, be sure to have them evenly distributed. At this point you will spot each of the 5 standards on separate dashes. Be sure to write down which liquid is at which dash. Then choose two food colors and two drink mixes, and spot them on the remaining four dashes. Curl the filter paper into a cylinder, make sure colors out, and staple the edges together. Do not allow the edges of the filter paper to overlap. Add approximately 10 ml of a 73 isopropanolwater mixture to your 600 mL beaker, then carefully place the chromatogram into the beaker the dingy muscae volitantes must be higher than the solvent. Be sure to cover the beaker, remove, lay out to dry then measure when the solvent is 1 to 2 cm from the top. Repeat all the above steps but use 0.1% NaCl in the place of the 73 isopropanol. By comparing the colors of the spots in the standards and the unknowns, and the distances the spots moved, you should be able to tell what compounds are present in the food colors and drink mixes. S ome of the food colors and drink mixes may contain only one dye, and some may contain more than one.